S1 In vitro symmetry breaking and motility for bead uniformly coated with ActA.  See methods for conditions.
S2 Computer simulation of symmetry breaking and motility (2D projections convolved with Gaussian)
S3 3D view of simulation showing links colored by tensile stress (color bar range represents zero to breakage stress)
S4 3D view of simulation, viewed from front of bead
S5 Fluorescence Speckle Microscopy movie of in vitro symmetry breaking
S6a Shell deformations during symmetry breaking (circumferential) 
S6b Shell deformations during symmetry breaking (radial)
S7 Strain buildup and release by link breakage. Images show node tracks, link breaks and transverse forces. Graph on right shows corresponding transverse and radial link force buildup and broken links as functions of distance from the surface of the bead
S8 Strain buildup and release by link breakage, showing compressive and tensile components
S9 Strain buildup and release by link breakage, showing the circumferential repulsion at the bead surface is distorted due to surface artifact
S10 Link breaks during symmetry breaking shown in 3 orthogonal views. Rupture is primarily a straightline crack
S11 Link breaks during smooth motility localize to outer network towards front of bead
S12 Fluorescence Speckle Microscopy movie of in vitro smooth motility
S13a Network deformations during smooth motion (circumferential). Note, for the statistics, only lines that are within the tail were included (circumferential lines spanning the rip were excluded)
S13b Network deformations during smooth motion (radial). Note, for the statistics, only lines that are within the tail were included (radial lines that open around the bead were excluded)
S14a Symmetry breaking and motility for less elastic network (x view, same run as S14b) (Parameters defaults except: NODE_REPULSIVE_MAG=5.0 LINK_BREAKAGE_FORCE=2.0 LINK_FORCE=4.0)
S14b Symmetry breaking and motility for less elastic network. (y view, same run as S14a) (Parameters defaults except: NODE_REPULSIVE_MAG=5.0 LINK_BREAKAGE_FORCE=2.0 LINK_FORCE=4.0)
S15 Symmetry breaking and motility for less elastic network with network contraction prevented (Parameters defaults except: NODE_REPULSIVE_MAG=5.0 LINK_BREAKAGE_FORCE=2.0 LINK_FORCE=4.0, nodes fixed 2xradius from bead)
S16 Simulation of half coated capsule-shaped bead moves lengthwise. Red lines show network trajectory
S17 Simulation of evenly coated capsule-shaped bead breaking symmetry sideways
S18 Simulation of evenly coated ellipsoidal bead breaking symmetry sideways (3D view)
S19 Simulation of evenly coated ellipsoidal bead breaking symmetry and moves sideways